Data on the presence of diarrheagenic Escherichia coli pathotypes (DEPs) in alfalfa sprouts and correlations between the presence of coliform bacteria (CB), fecal coliforms (FC), E. coli, DEPs, and Salmonella in alfalfa sprouts are not available. The presence of and correlations between CB, FC, E. coli, DEPs, and Salmonella in alfalfa sprouts were determined. One hundred sprout samples were collected from retail markets in Pachuca, Hidalgo State, Mexico. The presence of indicator bacteria and Salmonella was determined using conventional culture procedures. DEPs were identified using two multiplex PCR procedures. One hundred percent of samples were positive for CB, 90% for FC, 84% for E. coli, 10% for DEPs, and 4% for Salmonella. The populations of CB ranged from 6.2 up to 8.6 log CFU/g. The FC and E. coli concentrations were between , 3 and 1,100 most probable number (MPN)/g. The DEPs identified included enterotoxigenic E. coli (ETEC; 2%), enteropathogenic E. coli (EPEC; 3%), and Shiga toxin–producing E. coli (STEC; 5%). No E. coli O157:H7 strains were detected in any STEC-positive samples. In samples positive for DEPs, the concentrations ranged from 210 to 240 MPN/g for ETEC, 28 to 1,100 MPN/g for EPEC, and 3.6 to 460 MPN/g for STEC. The Salmonella isolates identified included Salmonella enterica serotype Typhimurium in three samples and Salmonella enterica serotype Enteritidis in one. STEC and Salmonella Typhimurium were identified together in one sample. Positive correlations were observed between FC and E. coli, between FC and DEPs, and between E. coli and DEPs. Negative correlations occurred between CB and DEPs and between CB and Salmonella. Neither FC nor E. coli correlated with Salmonella in the sprout samples. To our knowledge, this is the first report of ETEC, EPEC, and STEC isolated from alfalfa sprouts and the first report of correlations between different indicator groups versus DEPs and Salmonella.
This study investigated the bacterial contamination levels in ready-to-eat fresh raw beef, Saeng-go-gi in Korean, sold in restaurants. A total of 462 samples were analyzed by performing an aerobic bacterial plate count, a coliform count, and an Escherichia coli O157:H7 count. Aerobic bacterial plate counts of fresh raw beef obtained from Seoul, Cheonan, Daegu, Gunsan, and Gwangju retail store restaurants were 6.46, 6.89, 6.39, 6.58, and 6.67 log CFU/g, respectively, and coliforms were 4.05, 4.97, 4.76, 3.62, and 3.32 log CFU/g, respectively. Among the 462 assessed samples, suspected E. coli O157:H7 colonies were found in 32, 24, 20, 22, and 16 samples obtained from Seoul, Cheonan, Daegu, Gunsan, and Gwangju, respectively. The identity of these isolated colonies was further assessed by using a latex agglutination kit. The agglutination assay data showed that the isolates were not E. coli O157:H7. The data from this study could be used to design better food handling practices for reducing foodborne illnesses linked to fresh raw beef consumption.
The food recall warning issued February 25, 2015 has been updated to include additional product information. This additional information was identified during the ongoing food borne illness outbreak investigation in Ontario.
Lady York Foods is recalling sliced Mortadella products from the marketplace due to possible Listeria monocytogenes contamination. Consumers should not consume the recalled products.
If other high-risk products are recalled, the CFIA will notify the public through updated Food Recall Warnings.
The following products were sold from Lady York Foods, 2939 Dufferin St., North York, Ontario.
This recall applies to all Mortadella products sliced and sold from the deli counter at Lady York Foods from December 2, 2014 to February 24, 2015. Consumers who are unsure if they have purchased an affected product are advised to contact the retailer.
996660 Ontario Ltd. is recalling Monticello brand Mortadella, product of Italy, from the marketplace due to possible Listeria monocytogenes contamination. Consumers should not consume and retailers, restaurants and institutions should not to sell or use the recalled product described below.
Starting on November 13, 2014, this product was sold clerk-served from deli counters with or without a label or coding, and some product packages may not bear the same brand or product name as described above, or a brand at all. Consumers who are unsure if they have purchased the affected product are advised to contact their retailer.
|Brand Name||Common Name||Size||Code(s) on Product||UPC|
|Monticello||La Mortadella del Cuore – Mortadella||Variable||LOT: 044293
BEST BEFORE: 03/19/2015